Bulk RNA Sequencing | Bruhaspathi Institute of Biosciences

Bulk RNA Sequencing Services

Comprehensive RNA-Seq solutions — from RNA isolation and sequencing to high-quality analysis and publication-ready results.

What’s Included in Your Project

1. RNA Quality & Library Preparation

RNA extraction, quality assessment (Bioanalyzer/TapeStation), and cDNA library construction using Illumina TruSeq or NEBNext kits.

2. Sequencing

Next-generation sequencing (Illumina platform) providing optimal coverage, accuracy, and data quality for reliable transcriptome profiling.

3. Bioinformatics Analysis

Quality Control (FastQC, Trimmomatic)
Read Alignment (HISAT2 / STAR)
Transcript Quantification (FeatureCounts / Salmon)
Differential Expression (DESeq2 / edgeR)
Functional Enrichment (GO / KEGG)

4. Result Delivery & Publication Support

You receive full analysis outputs — expression matrices, volcano plots, PCA, heatmaps, and enriched pathways — along with manuscript and figure preparation support.

Deliverables

Raw FASTQ files (if sequencing performed)
Quality control and alignment reports
Gene expression matrix and DE analysis
Functional annotation and pathway results
Publication-ready visualizations and summary report (PDF)

Flexible Project Options

You can begin your RNA-Seq project with our complete service covering sample isolation, sequencing, and analysis — or opt for our bioinformatics-only package if you already have raw sequencing data.

Ready to Begin Your RNA-Seq Journey?

Let Bruhaspathi handle your sequencing or bioinformatics needs start from sample isolation or analysis directly.