DNA Methylation Analysis | Bruhaspathi Institute of Biosciences

What’s Included in Your Project

1. Sample Preparation & Bisulfite Conversion

High-quality DNA isolation, quantification, and bisulfite conversion using industry-standard protocols ensuring optimal methylation detection efficiency.

*Currently available on request or collaborative basis*

2. Library Preparation & Sequencing

Construction of bisulfite sequencing libraries (WGBS / RRBS) followed by paired-end sequencing on Illumina platforms for genome-wide CpG coverage.

*Currently available on request or collaborative basis*

3. Bioinformatics Pipeline

Quality Control (FastQC, Trim Galore)
Bisulfite Read Alignment (Bismark / BS-Seeker2)
Methylation Calling and Coverage Estimation
Differential Methylation Analysis (DMRfinder / methylKit)
Annotation & Integration with Gene Expression

4. Result Interpretation & Publication Support

Delivery of high-resolution methylation maps, DMRs, and integrative plots linking epigenetic changes to biological pathways — with figure and manuscript support.

Deliverables

Raw FASTQ and processed BAM files (if sequencing included)
Methylation calling summary and CpG coverage files
Lists of Differentially Methylated Regions (DMRs)
Genome annotation and visualization (IGV, Circos plots)
Publication-ready summary report (PDF) and pathway insights

Flexible Project Options

You can begin your methylation study with our complete wet-lab and analysis service — from DNA isolation to sequencing — or opt for a bioinformatics-only analysis if you already have bisulfite sequencing data.

Bruhaspathi Institute of Biosciences

DNA Methylation Analysis Services