Shotgun Metagenomics | Bruhaspathi Institute of Biosciences

Shotgun Metagenomics Services

Explore complex microbial communities with high-resolution shotgun metagenomics — from sample prep to comprehensive analysis and publication-ready results.

What’s Included in Your Project

1. Sample Collection & DNA Extraction

Extraction of high-quality DNA from diverse environmental or clinical samples for comprehensive microbial profiling.

*Currently available on request or collaborative basis*

2. Library Preparation & Sequencing

Shotgun metagenomic library construction followed by Illumina paired-end sequencing for deep coverage.

*Currently available on request or collaborative basis*

3. Bioinformatics Analysis

Quality Control and Trimming (FastQC, Trimmomatic)
Host Read Removal (Bowtie2)
Taxonomic Classification (Kraken2, MetaPhlAn3)
Functional Annotation (HUMAnN3, KEGG / eggNOG)
Assembly & Binning (MEGAHIT, MetaBAT2) if requested

4. Result Delivery & Publication Support

Comprehensive reports with taxonomic profiles, functional pathways, diversity metrics, and publication-ready visualizations.

Deliverables

Raw FASTQ files (if sequencing performed)
Processed taxonomic and functional tables
Assembly and binning results (if performed)
Diversity and functional pathway summaries
Publication-ready figures and summary report (PDF)

Flexible Project Options

You can start with our full wet-lab and sequencing service or choose bioinformatics-only analysis if you already have shotgun sequencing data.

Ready to Explore Microbial Communities in Detail?

Partner with Bruhaspathi for high-resolution shotgun metagenomics — start from sample collection or bring your sequencing data.