Shotgun Metagenomics Services
Explore complex microbial communities with high-resolution shotgun metagenomics — from sample prep to comprehensive analysis and publication-ready results.
What’s Included in Your Project
1. Sample Collection & DNA Extraction
Extraction of high-quality DNA from diverse environmental or clinical samples for comprehensive microbial profiling.
*Currently available on request or collaborative basis*
2. Library Preparation & Sequencing
Shotgun metagenomic library construction followed by Illumina paired-end sequencing for deep coverage.
*Currently available on request or collaborative basis*
3. Bioinformatics Analysis
- Quality Control and Trimming (FastQC, Trimmomatic)
- Host Read Removal (Bowtie2)
- Taxonomic Classification (Kraken2, MetaPhlAn3)
- Functional Annotation (HUMAnN3, KEGG / eggNOG)
- Assembly & Binning (MEGAHIT, MetaBAT2) if requested
4. Result Delivery & Publication Support
Comprehensive reports with taxonomic profiles, functional pathways, diversity metrics, and publication-ready visualizations.
Deliverables
Raw FASTQ files (if sequencing performed)
Processed taxonomic and functional tables
Assembly and binning results (if performed)
Diversity and functional pathway summaries
Publication-ready figures and summary report (PDF)
Flexible Project Options
You can start with our full wet-lab and sequencing service or choose bioinformatics-only analysis if you already have shotgun sequencing data.
Ready to Explore Microbial Communities in Detail?
Partner with Bruhaspathi for high-resolution shotgun metagenomics — start from sample collection or bring your sequencing data.