DNA Methylation Analysis Services
Comprehensive methylation profiling — from bisulfite conversion and sequencing to in-depth bioinformatics and publication-ready results.
What’s Included in Your Project
1. Sample Preparation & Bisulfite Conversion
High-quality DNA isolation, quantification, and bisulfite conversion using industry-standard protocols ensuring optimal methylation detection efficiency.
*Currently available on request or collaborative basis*
2. Library Preparation & Sequencing
Construction of bisulfite sequencing libraries (WGBS / RRBS) followed by paired-end sequencing on Illumina platforms for genome-wide CpG coverage.
*Currently available on request or collaborative basis*
3. Bioinformatics Pipeline
- Quality Control (FastQC, Trim Galore)
- Bisulfite Read Alignment (Bismark / BS-Seeker2)
- Methylation Calling and Coverage Estimation
- Differential Methylation Analysis (DMRfinder / methylKit)
- Annotation & Integration with Gene Expression
4. Result Interpretation & Publication Support
Delivery of high-resolution methylation maps, DMRs, and integrative plots linking epigenetic changes to biological pathways — with figure and manuscript support.
Deliverables
Raw FASTQ and processed BAM files (if sequencing included)
Methylation calling summary and CpG coverage files
Lists of Differentially Methylated Regions (DMRs)
Genome annotation and visualization (IGV, Circos plots)
Publication-ready summary report (PDF) and pathway insights
Flexible Project Options
You can begin your methylation study with our complete wet-lab and analysis service — from DNA isolation to sequencing — or opt for a bioinformatics-only analysis if you already have bisulfite sequencing data.
Ready to Explore Epigenetic Signatures?
Partner with Bruhaspathi for precise methylation analysis — begin from DNA isolation or choose computational services directly.
16S Metagenomics Services
Explore microbial diversity with our end-to-end 16S rRNA sequencing and bioinformatics analysis services — from sample to publication-ready insights.
What’s Included in Your Project
1. Sample Collection & DNA Extraction
High-quality microbial DNA extraction from your samples using optimized kits and protocols.
*Currently available on request or collaborative basis*
2. 16S rRNA Library Preparation & Sequencing
Amplification of 16S V3-V4 regions and paired-end sequencing on Illumina platforms for accurate microbial profiling.
*Currently available on request or collaborative basis*
3. Bioinformatics Analysis
- Quality Control and Filtering (FastQC, DADA2)
- OTU/ASV Clustering and Taxonomic Assignment (QIIME2, SILVA/Greengenes)
- Alpha and Beta Diversity Analysis
- Differential Abundance and Functional Prediction (PICRUSt2)
4. Result Delivery & Publication Support
Comprehensive reports including taxonomic profiles, diversity metrics, heatmaps, PCoA plots, and publication-ready figures.
Deliverables
Raw FASTQ files (if sequencing performed)
Processed OTU/ASV tables
Taxonomic assignment summary
Diversity and differential abundance reports
Publication-ready figures and summary report (PDF)
Flexible Project Options
You can start with our full experimental service or choose bioinformatics-only analysis if you already have 16S sequencing data.
Ready to Explore Microbial Communities?
Partner with Bruhaspathi for high-quality 16S metagenomics analysis — start from sample collection or bring your sequencing data.
AMR Prediction Services for Pure Cultures
Comprehensive antimicrobial resistance prediction from bacterial isolates — from genome sequencing to bioinformatics analysis and reporting.
What’s Included in Your Project
1. Bacterial Culture & DNA Extraction
High-quality genomic DNA extraction from pure bacterial cultures using standardized protocols.
*Currently available on request or collaborative basis*
2. Whole Genome Sequencing
Illumina-based sequencing for accurate, high-coverage bacterial genomes.
*Currently available on request or collaborative basis*
3. Bioinformatics AMR Analysis
- Quality Control and Read Trimming (FastQC, Trimmomatic)
- Genome Assembly (SPAdes)
- AMR Gene Identification (ResFinder, CARD, AMRFinderPlus)
- Resistance Prediction and Phenotype Correlation
- Optional Plasmid & Mobile Element Analysis
4. Report & Publication Support
Comprehensive reports including detected AMR genes, predicted resistance phenotypes, and publication-ready summary tables and figures.
Deliverables
Raw FASTQ files (if sequencing performed)
Genome assembly and QC reports
AMR gene and resistance prediction tables
Plasmid/mobilome summary (if included)
Publication-ready figures and summary report (PDF)
Flexible Project Options
You can start with our full experimental service or choose bioinformatics-only analysis if you already have bacterial genome sequencing data.
Ready to Predict Antimicrobial Resistance?
Partner with Bruhaspathi to identify resistance genes and phenotypes from your bacterial isolates.
Personalized Genomics & Data Curation Services
Specialized curation and analysis for personalized genomics, nutrigenomics, pharmacogenomics, and other bioinformatics services — from raw data to actionable insights.
What’s Included in Your Project
Data Collection & Quality Assessment
Collection of raw genomic or exome data (FASTQ, VCF) and quality assessment to ensure high-confidence input for downstream curation.
2. Data Curation & Annotation
Standardized curation including variant filtering, annotation, mapping to known databases (ClinVar, PharmGKB, SNPedia), and trait association.
3. Personalized Genomics Interpretation
- Polygenic risk scoring for complex traits
- Nutrigenomics & diet-related trait analysis
- Pharmacogenomics and drug response prediction
- Health and lifestyle trait correlations
- Optional disease susceptibility and carrier screening
4. Report & Consultation Support
Delivery of curated, comprehensive reports with actionable insights, visualizations, and expert consultation for interpretation and publication if required.
Deliverables
Curated genomic variant datasets (VCF / Excel)
Trait association tables and polygenic risk scores
Dietary and pharmacogenomic recommendations
Visualizations (plots, heatmaps, summary charts)
Full report in PDF with optional manuscript support
Flexible Project Options
We provide complete curation and interpretation services or customized analysis packages for researchers with existing genomic data.
Ready to Unlock Insights from Your Genomic Data?
Partner with Bruhaspathi for expert personalized genomics curation and interpretation.
Shotgun Metagenomics Services
Explore complex microbial communities with high-resolution shotgun metagenomics — from sample prep to comprehensive analysis and publication-ready results.
What’s Included in Your Project
1. Sample Collection & DNA Extraction
Extraction of high-quality DNA from diverse environmental or clinical samples for comprehensive microbial profiling.
*Currently available on request or collaborative basis*
2. Library Preparation & Sequencing
Shotgun metagenomic library construction followed by Illumina paired-end sequencing for deep coverage.
*Currently available on request or collaborative basis*
3. Bioinformatics Analysis
- Quality Control and Trimming (FastQC, Trimmomatic)
- Host Read Removal (Bowtie2)
- Taxonomic Classification (Kraken2, MetaPhlAn3)
- Functional Annotation (HUMAnN3, KEGG / eggNOG)
- Assembly & Binning (MEGAHIT, MetaBAT2) if requested
4. Result Delivery & Publication Support
Comprehensive reports with taxonomic profiles, functional pathways, diversity metrics, and publication-ready visualizations.
Deliverables
Raw FASTQ files (if sequencing performed)
Processed taxonomic and functional tables
Assembly and binning results (if performed)
Diversity and functional pathway summaries
Publication-ready figures and summary report (PDF)
Flexible Project Options
You can start with our full wet-lab and sequencing service or choose bioinformatics-only analysis if you already have shotgun sequencing data.
Ready to Explore Microbial Communities in Detail?
Partner with Bruhaspathi for high-resolution shotgun metagenomics — start from sample collection or bring your sequencing data.